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BioSpec 50-ml ice-jacketed bead beater
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Enzo Biochem suc-llvy-amc
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Corning Life Sciences individual optical connectors 64
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Becton Dickinson pst petri dish
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
Microwave Oven Sears Kenmore Model 85055, supplied by Sears Canada Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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USA Scientific Inc 50-ml falcon tube
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Promega automatic extractor maxwell csc dna blood kit
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Thermo Fisher pcr buffer
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Promega deoxynucleotide triphosphates (dntp)
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Thermo Fisher template dna
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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TwistDx Inc twistamp® basic kit
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Becton Dickinson matrigel
(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of <t>Suc-LLVY-AMC</t> in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
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Image Search Results


(A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of Suc-LLVY-AMC in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.

Journal: bioRxiv

Article Title: Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons

doi: 10.1101/629295

Figure Lengend Snippet: (A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of Suc-LLVY-AMC in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.

Article Snippet: The measurement was carried out in a total volume of 100 μL in 96 well plates with 50 μM Suc-LLVY-AMC (BML-P802, Enzo Lifesciences) in assay buffer (25 mM HEPES, 0.5 mM EDTA, 0.05% NP-40 Tergitol and 0.01% SDS) using 5, 15 and 25 μg of protein supernatant.

Techniques: Mass Spectrometry, Activity Assay