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Promega
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Image Search Results
Journal: bioRxiv
Article Title: Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons
doi: 10.1101/629295
Figure Lengend Snippet: (A) Experimental outline: mass spectrometry was performed in Day 2 neurons and proteasome activity tests were performed in Day 2 and Day 5 living neurons. (B) Unbiased mass spectrometry revealed that iCrMNs contain higher levels of all proteasome 20S core subunits but not 19S regulatory subunits (n=2). (C) iCrMNs have a higher proteasome activity than iSpMNs measured by hydrolysis rate of Suc-LLVY-AMC in living cells (n=6, mean ± SEM). n = biological replicates; statistical analysis was performed by student’s t-test, * p<0.05, ** p<0.01, *** p<0.001.
Article Snippet: The measurement was carried out in a total volume of 100 μL in 96 well plates with 50 μM
Techniques: Mass Spectrometry, Activity Assay